Aridia HLA-B*27 Real-time PCR Kit

PRODUCT DESCRIPTION

HLA B27 mutation Real-time PCR Kit constitutes a ready-to-use system for the detection of the HLA-B27 genotype using polymerase chain reaction (PCR). It contains reagents and enzymes for the specific amplification for the direct detection of the specific amplicon in fluorescence channels FAM. In addition, it contains an endogenous control amplification system amplifies human gene detected in HEX channel which identify possible PCR inhibition and DNA purification. External mutation positive control is supplied to assist the run

STORAGE

1. The kit is shipped on gel ice. Upon arrival, all components should be stored in -20°C. They are stable until the expiration date stated on the label. 
2. Repeated thawing and freezing should be avoided, as this might affect the performance of the assay. 
3. If the reagents are to be used only intermittently, they should be frozen in aliquots. Storage at 2 to 8°C should not exceed a period of 5 hours

SPECIFICITY

HLA-B27 primer and probe have been designed for the specific and exclusive in vitro detection of HLA-B27 genotypes. The Primer Probe can able to detect all the subtypes ranging from B27:01 to B27:73. The target sequence is highly conserved and has previously been shown to be a good genetic marker for the detection of HLA-B27 genotyping. The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.