Aridia JAK2 Real-time PCR Kit

PRODUCT DESCRIPTION

JAK2 V617F mutation Real-time PCR Kit constitutes a ready-to-use system for the detection of the point mutation 1849 (G→T) V617F in the JAK2 gene occurs at high frequency in several chronic myeloproliferative diseases using polymerase chain reaction (PCR). It contains reagents and enzymes for the specific amplification for the direct detection of the specific amplicon in fluorescence channels FAM. In addition, it contains an endogenous control amplification system amplifies human gene detected in HEX channel which identify possible PCR inhibition and DNA purification. External mutation positive control is supplied to assist the run.

STORAGE

1. The kit is shipped on gel ice. Upon arrival, all components should be stored in -20°C. They are stable until the expiration date stated on the label. 
2. Repeated thawing and freezing should be avoided, as this might affect the performance of the assay. 
3. If the reagents are to be used only intermittently, they should be frozen in aliquots. Storage at 2 to 8°C should not exceed a period of 5 hours

SPECIFICITY

JAK2 mutation primer and probe have been designed for the specific and exclusive in vitro detection of 1849 (G→T), in the JAK2 human gene [GenBank accession no. NM_004972]. The primers and probe sequences in this kit have 100% homology with clinically relevant reference sequences based on a comprehensive bioinformatics analysis.