TRUSTwell Thyroid Stimulating Hormone (TSH) ELISA

TEST PRINCIPLE

The TSH ELISA Kit is a solid-phase enzyme-linked immunosorbent assay based on the principle of antibody sandwich technique for the quantitative determination of TSH concentration in human serum. 

The TSH ELISA Kit is composed of two key components: 
1) Solid microwells pre-coated with monoclonal anti-TSH antibody, specific for the β-subunit. 
2) Liquid conjugate composed of monoclonal anti-intact TSH antibody conjugated with horse radish peroxidase (HRP-anti-TSH Conjugate). 

During the assay, the test specimen and HRP-anti-TSH Conjugate, are incubated simultaneously with the coated microwells. The TSH, if present in the specimen, binds to both the anti- β TSH antibody on the microwell surface and the anti-TSH antibody in the HRP Conjugate, forming an antibody sandwich immunocomplex. Unbound conjugate is then removed by washing. The presence of the conjugate complex is shown by the development of a blue color upon an additional incubation with the substrate. The reaction is terminated with Stop Solution, and the absorbance determined using a spectrophotometer at 450/620-690 nm. A standard curve is generated by plotting the absorbance at 450/620-690 nm wavelength versus the respective TSH concentration for each standard. The concentration of TSH in the samples is then determined directly from this curve.

STORAGE AND STABILITY

All reagents except the concentrated wash buffer are ready to use as supplied. Store all components at 2-8°C. Do not freeze. Avoid strong light. Reseal the microwells after removing the desired number of wells. Ensure that the reagents are brought to room temperature before opening. All the reagents are stable through the expiration date printed on the label if not opened. Place unused wells in the resealable bag with desiccant and return to 2-8°C