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The TRUSTwell HIV 1+2 Ab ELISA Kit is a solid phase enzyme linked immunosorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma. It is intended for professional use only as an aid in the diagnosis of infection with HIV-1 and HIV-2 viruses. Any reactive specimen with the TRUSTwell HIV 1+2 Ab ELISA Kit must be confirmed with alternative testing method(s) and clinical findings
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INTRODUCTION
Human immunodeficiency virus type I and type II (HIV1+2) are enveloped single stranded RNA positive virus. The causative relationship between HIV1+2 virus and acquired immunodeficiency syndrome (AIDS) has been established over decades. HIV-1 has been isolated from patients with AIDS and AIDS-related complex, and from healthy individuals with a high risk for developing AIDS (1). HIV-2 has been isolated from West African AIDS patients and from seropositive asymptomatic individuals.
Infection with HIV induces the immune system to produce antibodies against viral proteins from different parts of the HIV genome, ENV, GAG and POL. Diagnosis of anti-HIV seropositivity is based on the detection of these specific antibodies. The TRUSTwell HIV 1+2 Ab ELISA Kit is a third generation of HIV kits for the qualitative detection of the presence of anti-HIV-1 and HIV-2 antibodies.
STORAGE AND STABILITY
All reagents except the concentrated wash buffer are ready to use as supplied. Refrigerate all the reagents immediately after use. Reseal the microwells after removing the desired number of wells. Ensure that the reagents are brought to room temperature before opening. All the reagents are stable through the expiration date printed on the label if not opened. Do not freeze the kit or expose the kit over 8°C.
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