TRUSTwell Dengue IgG ELISA

TEST PRINCIPLE

The Dengue IgG ELISA Kit is a solid-phase enzyme-linked immunosorbent assay based on the principle of the capture immunoassay methodology for the detection of IgG anti-dengue virus in human serum or plasma. 

The Dengue IgG ELISA Kit is composed of two key components: 
1) Solid microwells pre-coated with mouse monoclonal anti-human IgG antibody. 
2) Conjugate composed of biotinylated dengue antigens and avidin HRP-conjugates. 

During the assay, the test specimen is first incubated in the coated microwell. IgG antidengue, if present in the specimen, binds to the anti-human IgG antibodies coated on the microwell surface, and any unbound specimen is then removed by a wash step. During a second incubation with Biotinylated dengue antigens, the IgG anti-dengue absorbed on the surface of microwell binds with dengue antigen, forming a complex. Unbound antigens are then removed by washing. After addition of Streptavidin HRP conjugate, it binds with biotinylated antigen and forming complex with HRP conjugate. After addition of the TMB substrate, the presence of the conjugate complex is shown by development of a blue color resulting from a reaction between the enzyme and substrate. This reaction is then quenched by addition of the Stop Solution, and the absorbance value for each microwell is determined using a spectrophotometer at 450/620-690 nm.

STORAGE AND STABILITY

All reagents except Dengue Antigen Mix and concentrated Wash Buffer are ready to use as supplied. Store all components at 2-8°C. Do not freeze. Ensure that the reagents are brought to room temperature before opening. Return all reagents requiring storage at 2-8°C to refrigeration immediately after use. Re-seal the unused microwells in provided Ziploc bag with desiccant. All reagents are stable through the expiration date printed on the label if not opened. Once opened, the kit is stable for 8 weeks at 2-8°C, or until the labeled expiration date, whichever is earlier.