TRUSTwell Anti-Mullerian Hormone (AMH) ELISA

TEST PRINCIPLE

The TRUSTwell AMH ELISA is a quantitative solid-phase enzyme-linked immunosorbent assay, based on the principle of antibody sandwich technique, for the measurement of AMH concentration in human serum or plasma

The TRUSTwell AMH ELISA is comprised of three key components:

1. Solid microwells pre-coated with AMH antibody. 
2. AMH calibrators and controls. 
3. AMH Biotin-Ab reagent and AMH enzyme reagent comprised of horse radish peroxidase conjugated to streptavidin.

During the assay, the calibrator, control, or test specimen as well as the biotin reagent containing the Anti-AMH-Ab-Biotin is added to the AMH-antibody coated microwell. During the 60-minute incubation, the AMH in the test specimen binds to both the coated antibody and the Biotin-Ab forming the sandwich-complex. Unbound material is then removed by washing. In the next step, HRP conjugated streptavidin is added, then washed after incubation. Next, TMB single Substrate is added and the presence of the HRP bound to microwell surface is shown by the development of a blue color. The reaction is then terminated with the Stop Solution and the optical density (OD) is determined using a spectrophotometer at 450/620-690 nm. The color intensity reflects the amount of Ab-HRP bound to the AMH-Ab coated microwell, and is directly proportional to the amount of AMH in the control or test specimen.

STORAGE AND STABILITY

All reagents except the concentrated wash buffer, calibrators and controls are ready for use as supplied. Store all components at 2-8°C. Ensure that the reagents are brought to room temperature before opening. After removing the desired number of wells, place unused wells in the resealable foil pouch provided with desiccant and return to 2-8°C.