RecombiLISA Total Triiodothyronine (T3) ELISA

TEST PRINCIPLE

The RecombiLISA Total Triiodothyronine (T3) ELISA is a competitive, solid-phase enzyme-linked immunosorbent assay for the quantitative measurement of T3 concentration in human serum

The RecombiLISA Total Triiodothyronine (T3) is comprised of three key components: 

1) Solid microwells pre-coated with anti-T3 antibody, 
2) T3 Standards, 
3) Liquid conjugate comprised of horseradish peroxidase conjugated to T3 (T3 Enzyme Conjugate Reagent)

During the assay, the test specimen and the T3 Enzyme Conjugate Reagent are simultaneously incubated with the microwells coated with anti-T3 antibody. The T3 in the patient specimen will then compete with the T3 in the T3 Enzyme Conjugate Reagent for binding to the anti-T3 antibody coated on the microwell surface.

Unbound material is then removed by washing. The presence of the T3-HRP bound to the microwell surface is shown by the development of a blue color upon addition of the Substrate. The reaction is then terminated with Stop Solution and the absorbance is determined using a spectrophotometer at 450/620-690 nm. The color intensity reflects the amount of T3-HRP bound to the microwell surface, and is inversely proportional to the amount of T3 in the patient specimen, within the dynamic range of the assay.

STORAGE AND STABILITY

All reagents except the concentrated wash buffer are ready to use as supplied. Store all components at 2-8°C. Do not freeze. Avoid strong light. All the reagents are stable through the expiration date printed on the label if not opened. Ensure that the reagents are brought to room temperature before opening. Reseal the microwells after removing the desired number of wells. Place unused wells in the resealable plastic bag provided and return to 2-8°C