Elabscience AF (Total Aflatoxin) Elisa Kit

Test principle 
This kit uses Competitive-ELISA as the method for the quantitative detection. It can detect Total Aflatoxin (AF) in samples, such as grain, feed, edible oil, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard liquid and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.

Technical indicator
Reaction mode (Incubation time and temperature): 25℃, 30 min, 15 min.
Detection limit: Grain ---0.72 ppb; Feed, Chinese herbal medicine, Seasoning, Spice powder ---0.9 ppb; 
                            Edible oil---0.3 ppb; Muscle (beef, pork), Ham sausage---0.06 ppb; Milk---0.06 ppb.
Cross-reactivity: : Aflatoxin B1 (AFB1) ---100%; AFB2---39%; AFG1---100%; AFG2---13.2%; AFM1---6.6%; 
                                 AFM2--- < 1.0%.
Sample recovery rate: 90±30%.

Storage and expiry date
Store the kit at 2-8℃. Do not freeze any test kit components.
Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8℃.
Expiry date: expiration date is on the packing box.