DRG IGF-1 600 Elisa Kit

PRINCIPLE OF THE TEST
The DRG IGF-1 600 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. Patient samples, standards and controls are acidified and neutralized prior to the assay procedure. The microtiter wells are coated with a monoclonal antibody directed towards an antigenic site on the IGF-1 molecule. The pre-treated sample is incubated at room temperature with Conjugate (biotinylated IGF-1). The wells are washed and then incubated with Enzyme Complex (Streptavidin-HRP-Complex). After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of IGF-1 in the patient sample.

SPECIMEN COLLECTION AND PREPARATION
Serum can be used in this assay.
NOTE: In plasma significantly reduced values were observed.
Note: Samples containing sodium azide should not be used in the assay. In general it should be avoided to use haemolytic, icteric or lipaemic specimens. For further information refer to chapter “Interfering Substances”.
Specimen Collection
Serum: Collect blood by venipuncture (e.g. Sarstedt Monovette for serum), allow to clot, and separate serum by centrifugation at room temperature. Do not centrifuge before complete clotting has occurred. Patients receiving anticoagulant therapy may require increased clotting time.

Calculation of Results
Calculate the average absorbance values for each set of standards, controls and patient samples.
Using semi-logarithmic graph paper, construct a standard curve by plotting the mean absorbance obtained from each standard against its concentration with absorbance value on the vertical (Y) axis and concentration on the horizontal (X) axis.
Using the mean absorbance value for each sample determine the corresponding concentration from the standard curve.
Automated method: The results in the Instructions for Use have been calculated automatically using a 4 Parameter curve fit (4 Parameter Rodbard or 4 Parameter Marquardt are the preferred methods). Other data reduction functions may give slightly different results.
The concentration of the samples can be read directly from this standard curve. Samples with concentrations higher than that of the highest standard have to be further diluted or reported as > 600 ng/mL. For the calculation of the concentrations this dilution factor has to be taken into account.
 

QUALITY CONTROL
Good laboratory practice requires that controls be run with each calibration curve. A statistically significant number of controls should be assayed to establish mean values and acceptable ranges to assure proper performance. 
It is recommended to use control samples according to state and federal regulations. The use of control samples is advised to assure the day to day validity of results. Use controls at both normal and pathological levels. 
The controls and the corresponding results of the QC-Laboratory are stated in the QC certificate added to the kit. The values and ranges stated on the QC sheet always refer to the current kit lot and should be used for direct comparison of the results. 
It is also recommended to make use of national or international Quality Assessment programs in order to ensure the accuracy of the results. Employ appropriate statistical methods for analysing control values and trends. If the results of the assay do not fit to the established acceptable ranges of control materials patient results should be considered invalid. 
In this case, please check the following technical areas: Pipetting and timing devices; photometer, expiration dates of reagents, storage and incubation conditions, aspiration and washing methods. 
After checking the above mentioned items without finding any error contact your distributor or DRG directly.