DRG Corticosterone Elisa Kit

PRINCIPLE OF THE TEST
The DRG Corticosterone ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. 
The microtiter wells are coated with a polyclonal antibody directed towards a unique antigenic site of the corticosterone molecule. During the first incubation, the corticosterone in the added sample competes for the binding to the coated antibody with the added conjugate molecule, which is a Corticosterone molecule conjugated to a horse-radish peroxidase. After incubation the unbound conjugate and sample are washed off. Following the addition of the substrate solution, the intensity of colour developed is inversely proportional to the concentration of corticosterone in the sample. After incubation of substrate solution, the colorimetric reaction is abruptly stopped by adding stop solution.

Storage Conditions 
When stored at 2 °C to 8 °C unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date. 
Opened reagents must be stored at 2 °C to 8 °C. Microtiter wells must be stored at 2 °C to 8 °C. Once the foil bag has been opened, care should be taken to close it tightly again. Opened kits retain activity for 8 weeks if stored as described above.

LIMITATIONS OF USE 
Reliable and reproducible results will be obtained when the assay procedure is performed with a complete understanding of the package insert instruction and with adherence to good laboratory practice. Any improper handling of samples or modification of this test might influence the results.

WARNINGS AND PRECAUTIONS
This kit is for in vitro diagnostic use only. For professional use only.
All reagents of this test kit which contain human serum or plasma have been tested and confirmed negative for HIV I/II, HBsAg and HCV by FDA approved procedures. All reagents, however, should be treated as potential biohazards in use and for disposal.
Before starting the assay, read the instructions completely and carefully. Use the valid version of instructions for use provided with the kit. Be sure that everything is understood.
The microplate contains snap-off strips. Unused wells must be stored at 2 °C to 8 °C in the sealed foil pouch and used in the frame provided.