DRG Aspergillus fumigatus IgG Elisa Kit

TEST PRINCIPLE 
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The wells are coated with antigen. Specific antibodies of the sample binding to the antigen coated wells are detected by a secondary enzyme conjugated antibody (E-Ab) specific for human IgG. After the substrate reaction the intensity of the color developed is proportional to the amount of IgG-specific antibodies detected. Results of samples can be determined directly using the standard curve.

SUMMARY AND EXPLANATION 
The most common pathogen of the genus Aspergillus is A. fumingatus occuring in hay, grain, rotten plants and bird faeces. The main opportunistic invasive fungal infections are the candidal mycosis followed by aspergillosis. In general infections with Aspergillus spp. are airborne. Because of the ubiquity of Aspergillus species it is difficult to decide between contamination by commensalism or a serious infection. Usually infection in humans occurs in already damaged tissues only. Aspergillus spp. can cause a chronic infection of paranasal sinus, eyes or lungs. Three types of lung-aspergillosis can be distinguished: acute infection (bronchial pneumonia; pneumonia), saprophytic aspergillom (compact reticulum of hyphae in the lungs) and allergic bronchopulmonal aspergillosis (mediated by IgE). Next to the ELISA the indirect Aspergillus hemagglutination test (Aspergillus HAT) can be performed to detect specific IgG and IgM antibodies. The HAT is not suitable as a screening test, however, because of its low sensitivity. In some high-risk patients it shows only low antibody titers.

STORAGE AND STABILITY 
The kit is shipped at ambient temperature and should be stored at 2 °C - 8 °C. Keep away from heat or direct sun light. The storage and stability of specimen and prepared reagents is stated in the corresponding chapters. The microtiter strips are stable up to the expiry date of the kit in the broken, but tightly closed bag when stored at 2 °C - 8 °C.

WARNINGS AND PRECAUTIONS
For in-vitro diagnostic use only. For professional use only.
Before starting the assay, read the instructions completely and carefully. Use the valid version of the package insert provided with the kit. Be sure that everything is understood.
In case of severe damage of the kit package please contact your supplier in written form, latest one week after receiving the kit. Do not use damaged components in test runs, but keep safe for complaint related issues.
Obey lot number and expiry date. Do not mix reagents of different lots. Do not use expired reagents.
Follow good laboratory practice and safety guidelines. Wear lab coats, disposable latex gloves and protective glasses where necessary.
Reagents of this kit containing hazardous material may cause eye and skin irritations. See MATERIALS SUPPLIED and labels for details. Material Safety Data Sheets for this product are available upon request.