DRG Aldosterone ELISA

PRINCIPLE OF THE TEST

The DRG Aldosterone ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the principle of competitive binding. The microtiter wells are coated with a monoclonal antibody (mouse) directed towards a unique antigenic site of the aldosterone molecule. During the first incubation, the aldosterone in the added sample competes with the added enzyme conjugate, which is aldosterone conjugated to horseradish peroxidase, for binding to the coated antibody. After a washing step to remove all unbound substances, the solid phase is incubated with the substrate solution. The colorimetric reaction is stopped by addition of stop solution, and optical density (OD) of the resulting yellow product is measured. The intensity of color is inversely proportional to the concentration of the analyte in the sample. A standard curve is constructed by plotting OD values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.

STORAGE AND STABILITY OF THE KIT

Unopened kits and reagents as well as opened reagents must be stored at 2 °C to 8 °C. 

The microtiter plate contains snap-off strips. Do not open the pouch of the wells until it reaches room temperature. Unused wells must be stored at 2 °C to 8 °C in the sealed foil pouch including the desiccant and used in the plate frame provided. Once the foil bag has been opened, care must be taken to close it tightly again.