DLD Nor - Metanephrine in Plasma - Elisa

Specimen Collection and Storage Plasma 
EDTA plasma should be used. 
Drugs, alcohol and tobacco as well as stress influence the catecholamine release. This may lead to false positive results for metanephrine and normetanephrine. If clinically acceptable, medication (i.e. L-Dopa, alpha-blocker, antidepressants, MAO inhibitor, etc.) should be stopped five days before blood collection. Patient should adhere to an at least four hours fasting; no tea, coffee, alcohol, nicotine and no strong physical activity. 
It is recommended to let the patient rest for 20 to 30 minutes after the venipuncture and before collecting the blood sample. 
The samples can be stored up to 6 hours at 2 - 8 °C. For a longer storage the samples must be frozen at -20 °C and are stable for at least 12 months. Repeated freezing and thawing should be avoided.

Calculation of Results 
Concentrations of the standards: See q.c. certificate. 
Conversion: 
Metanephrine: 1 pg / ml = 5.07 pmol / l 
Normetanephrine: 1 pg / ml = 5.46 pmol / l 
On a semilogarithmic graph paper the concentration of the standards (x-axis, logarithmic) are plotted against their corresponding optical density (y-axis, linear). Alternatively, the optical density of each standard and sample can be related to the optical density of the zero standard, expressed as the ratio OD/ODmax, and then plotted on the y-axis. 
A good fit is provided with 4 Parameter Logistic (alternatively Log-Logit or Cubic Spline). 
The concentration of the controls and samples can be read directly from the standard curve in pg / ml.

Warnings and Precautions 
For in-vitro diagnostic use only. For professional use only. 
All reagents of human origin used in this kit are tested for HIV I/II antibodies, HCV and HBsAg and found to be negative. However, because no test method can offer complete assurance that infectious agents are absent, these reagents should be handled as potentially biohazardous materials. 
Material of animal origin used in the preparation of the kit has been obtained from animals certified as healthy but these materials should be handled as potentially infectious. 
Obey lot number and expiry date. Do not mix reagents of different lots. Do not use expired reagents. 
By handling reagents, controls and samples follow good laboratory practice and safety guidelines. 
Wear lab coats, disposable gloves and protective glasses. 
Some components of this kit are containing hazardous reagents. These components are marked with the adequate hazard label. Further information: See 4. Contents of the Kit and the safety data sheet. 
Avoid contact with reagents. It may causes eye and skin irritations and chemical burns.