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Normetanephrine and metanephrine are physiologically formed from the catecholamines noradrenaline and adrenaline by the enzyme catechol-Omethyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in patients suffering from pheochromocytoma, ganglioneuroma and other neurogenic tumors.
The assay kit provides materials for the quantitative measurement of free normetanephrine and metanephrine in human EDTA plasma. Normetanephrine and metanephrine are separated from plasma proteins through addition of precipitation reagents and then are quantitatively acylated to their N-acylderivates.
Mon - Sat: 10AM - 06PM
STORAGE AND STABILITY
On arrival, store the kit at 2 - 8 °C. Once opened the kit is stable until its expiry date. For stability of the ready for use reagents: See vial labels. For stability and storage of prepared reagents refer to 6.1 Preparation of Reagents.
SPECIMEN COLLECTION AND STORAGE
Plasma
EDTA plasma should be used.
Drugs, alcohol and tobacco as well as stress influence the catecholamine release. This may lead to false positive results for metanephrine and normetanephrine. If clinically acceptable, medication (i.e. L-Dopa, alpha-blocker, antidepressants, MAO inhibitor, etc.) should be stopped five days before blood collection. Patient should adhere to an at least four hours fasting; no tea, coffee, alcohol, nicotine and no strong physical activity.
It is recommended to let the patient rest for 20 to 30 minutes after the venipuncture and before collecting the blood sample. The samples can be stored up to 6 hours at 2 - 8 °C. For a longer storage the samples must be frozen at -20 °C and are stable for at least 12 months. Repeated freezing and thawing should be avoided.
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