DLD Methylhistamine - 1 Elisa

Storage and Stability 
The kit is shipped at ambient temperature. Upon arrival, store the kit at 2 – 8 °C to keep it stable until its expiry date. Once opened the kit is stable until its expiry date. The shelf life of the ready-to-use reagents is indicated on the respective bottle label. For stability of prepared reagents refer to 6. Reagents must equilibrate to room temperature before use and refrigerated immediately after use.

Sample Collection 
Avoid repeated freezing and thawing of the samples. Spontaneous urine can be used for this test as well as collected urine. Collected urine: In this case the total volume of urine excreted during a 24- hours period should be collected and mixed in a single bottle containing 10 - 15 ml of 6 M hydrochloric acid (Warning: Observe hazard warnings) as preservative. Determine the total volume and take an aliquot for the measurement. For patients with suspected kidney disorders the creatinine concentration should be determined, too. Urine samples can be stored at -20 °C for at least 6 months.

Calculation of the Results 
On a semilogarithmic graph paper the concentration of the standards (10 / 30 / 100 / 300 / 1000 ng/ml) (x-axis, logarithmic) are plotted against their corresponding optical density (y-axis, linear). 

A good fit is provided with 4 Parameter Logistic (alternatively Log-Logit or Cubic Spline). 

The concentration of the controls and urine samples can be read directly from this standard curve by using their average optical density.