DLD Calretinin Elisa

Storage and Stability 
Upon arrival, store the kit at 2 – 8 °C to keep it stable until its expiry date. Once opened the kit is stable until its expiry date. 
The shelf life of the readyto-use reagents is indicated on the respective bottle label. For stability of prepared reagents refer to 6. Reagents must equilibrate to room temperature before use and refrigerated immediately after use.

Specimen Collection and Storage 
EDTA plasma and serum should be used. Haemolytic, icteric and lipaemic samples should not be used. 
The samples can be stored up to 24 hours at room temperature. For a longer storage (up to 24 months) the samples must be frozen at -20 °C. Repeated freezing and thawing of samples should be avoided.

Calculation of the Results 
The concentration of the standards (x-axis, logarithmic) are plotted against their corresponding optical density (y-axis, linear). 
When using evaluation software, the 4 Parameter Logistic Regression is recommended (alternatively, Log-Logit or Cubic Spline). 
Using their ODs, the concentration of the controls and samples can be read directly from this standard curve in ng/ml.