Calbiotech Troponin I ELISA

SUMMARY AND EXPLANATION 
Troponin is the inhibitory or contractile regulating protein complex of striated muscle. It is located periodically along the thin filament of the muscle and consists of three distinct proteins: Troponin I, Troponin C, and Troponin T. Likewise, the Troponin I subunit exists in three separate isoforms; two in fast-twitch and slow-twitch skeletal muscle fibers, and one in cardiac muscle. The cardiac isoform (cTnI) is about 40% dissimilar, has a molecular weight of 22,500 daltons, Cardiac Troponin I (cTnI) has been useful in the differential diagnosis of patients presenting to Emergency Departments (ED) with chest pain 18-20. Myocardial infarction is diagnosed when blood levels of sensitive and specific biomarkers, such as cardiac Troponin, the MB isoenzyme of creatine kinase (CK-MB), and myoglobin, are increased in a clinical setting of acute ischemia. The most recently described and preferred biomarker for myocardial damage is cardiac Troponin (I or T). The cardiac Troponins exhibit myocardial tissue specificity and high sensitivity. The level of cTnI remains elevated for a much longer period of time (6-10 days), thus providing for a longer window of detection of cardiac injury. Normal levels of cTn I in the blood are very low. After the onset of an AMI, cTnI levels increase substantially and are measurable in serum within 4 to 6 hours, with peak concentrations reached in approximately 12 to 24 hours after infarction. The cTnI Enzyme Immunoassay provides a rapid, sensitive, and reliable assay for the quantitative measurement of cardiac-specific Troponin I.

PRINCIPLE OF ASSAY 
The Calbiotech Inc., cTnI ELISA is based on solid phase sandwich ELISA method. The assay utilizes four monoclonal antibodies which recognize different and distinct Troponin I epitopes. The samples and conjugate reagent (anti-cTnI biotin & HRP) are added to the wells coated with Streptavidin. cTnI in the patient's serum binds to the Abs, forming a sandwich complex and simultaneously the complex is being immobilized on the plate through streptavidin-biotin interactions. Unbound protein and conjugate are washed off, through a washing step. Upon addition of the substrate, the intensity of color is proportional to the concentration of cTnI in the samples. A standard curve is prepared by relating the color intensity to the concentration of cTnI.

STORAGE AND STABILITY 
1. Store the kit at 2-8°C. 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose test reagents to heat, sun or strong light.