Calbiotech Neonatal Thyroxine (T4) ELISA

PRINCIPLE OF THE TEST 
The Calbiotech Inc., Neonatal Thyroxine ELISA is a competitive solid phase ELISA that uses a whole blood spot sample dried on WHATMAN type 903 filter paper. In the assay, streptavidin coated wells are incubated with dry blood spots (DBS), 50µl T4-HRP conjugate, and 50µl mouse anti-T4 MAb. T4-HRP conjugate competes with T4 molecules in the standards and samples for a fixed number of binding sites of the specific anti-T4 antibody. Thus, the amount of T4-peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of thyroxine in the specimen increases. Unbound T4 is then removed by the washing steps. TMB Substrate is added, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is spectrophotometrically measured at 450nm. A standard curve is prepared relating color intensity to the concentration of Thyroxine.

STORAGE ANDSTABILITY 
1. Store the kit at 2-8°C. 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose test reagents to heat, sun or strong light.