Calbiotech Neonatal Maple Syrup Urine Disease (N-MSUD) ELISA

PRINCIPLE OF THE ASSAY 
The MSUD Kit uses trichloroacetic acid (TCA) to extract Leucine and BCAAs from dried blood spot samples. After extraction, the eluted sample is combined with the enzyme reagent Leucine dehydrogenase. This enzyme reagent catalyzes the NAD-dependent oxidative deamination of Leucine and L-BCAAs to α-ketoisocaproate acid. The NADH produced reacts with a color reagent in which a tetrazolium salt gets reduced producing a distinct color.