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Calbiotech Neonatal Galactose ELISA

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The Calbiotech Galactose Screening Assay is an enzymatic colorimetric end-point method for the determination of total D-Galactose in dried blood spot specimens taken from newborn human infants as part of a newborn screening program.

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SKU:GL345N
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PRINCIPLE OF THE TEST 
The Calbiotech Galactose Kit uses trichloroacetic acid (TCA) to extract Galactose from dried blood spot samples. After extraction, the eluted sample is combined with the enzyme reagents Galactose Dehydrogenase and Alkaline Phosphatase . These enzyme reagents catalyzes the NAD-dependent oxidative reaction of Galactose to D-galactono-1,4-lactone + NADH + H+. The NADH produced reacts with a color reagent in which a tetrazolium salt gets reduced producing a distinct color.

STORAGE AND STABILITY 
1. Store the kit at 2-8ºC. 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose test reagents to heat, sun or strong light.

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