Calbiotech Free Beta-Human Chorionic Gonadotropin (Free β-hCG) ELISA

PRINCIPLE OF THE TEST 
The Calbiotech free β-hCG kit is a solid phase sandwich assay method based on streptavidin-biotin principle. Standards, samples, and the biotinylated anti-free β-hCG antibody reagent are added into wells coated with Streptavidin. Free β-hCG in the samples binds to the biotinylated antibody. Simultaneously, the biotinylated antibody binds to the Streptavidin coated plate. Unbound protein and excess biotin conjugated antibody are washed off by wash buffer. Upon the addition of Peroxidase (HRP) conjugated anti-free β-hCG antibody reagent, a sandwich complex is formed, where the free β-hCG being in between the two highly specific antibodies, labeled with biotin and HRP. Unbound protein and excess enzyme conjugated antibody reagent is washed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of free β-hCG in the samples. A standard curve is prepared relating color intensity to the concentration of free β-hCG.

STORAGE AND STABILITY 
1. Store the kit at 2-8°C. 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose test reagents to heat, sun or strong light