Calbiotech Folate ELISA

PRINCIPLE OF THE TEST 

The Folate test kit is a solid phase enzyme-linked immunoassay (ELISA), based on the principal of competitive binding. Streptavidin coated wells are co-incubated with extracted Folate standards, controls, samples, Folate Binding Protein-Biotin conjugate and Folate-Enzyme conjugate at room temperature for 60 minutes. During the incubation, the folate-enzyme conjugate and the folate in the sample, standard, or quality control serum will compete to bind to the biotin-labeled folate binding protein. After the 60 minute incubation, all unbound conjugates are then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance. The color intensity is inversely proportional to the amount of Folate in the sample. The total assay procedure run time is 1.25 hours