Calbiotech CA19-9 ELISA

SUMMARY AND EXPLANATION 
A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA19-9 and CA19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistologic distribution of CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recently reports indicates that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA19-9 is suggestive of gallbladder neoplasm in the setting of inflammatory gallbladder disease. This tumor-associated antigen may also be elevated in some non-malignant conditions. Research studies demonstrate that serum CA 19-9 values may have utility in monitoring subjects with the above-mentioned diagnosed malignancies. It has been shown that a persistent elevation in serum CA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA 19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA 19-9 value may be indicative of a favorable prognosis and good response to treatment.

PRINCIPLE OF THE TEST 
The Ca19-9 Elisa Test Is An Adapted Solid Phase Sequential Sandwich Elisa. Samples And Biotinylated Monoclonal Antibody Are Added To Wells Coated With Streptavidin. Ca19-9 In The Patient Sample Binds To Biotinylated Capture Antibody. The Biotinylated Antibody Simultaneously Binds To The Streptavidin Coated Plate. After A Wash Step, Anti-Ca19-9–Hrp Enzyme Conjugate Is Added And Forms A Sandwich Around Captured Ca19-9. Unbound Antibodies Are Washed Off. Tmb Substrate Is Added Resulting In The Development Of A Blue Color. The Concentration Of Ca19-9 Is Directly Proportional To The Color Intensity Developed. A Standard Curve Is Generated Relating Color Intensity To Ca19-9 Concentration.

STORAGE AND STABILITY 
1. Store the kit at 2 - 8°C. 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose reagents to heat, sun, or strong light.