Calbiotech Bio-intact PTH [Parathyroid Hormone] ELISA

PRINCIPLE OF THE TEST 
The Calbiotech Bio-intact PTH (BiPTH) immunoassay is an adapted two-site sandwich ELISA. In this assay, standards and patient samples are simultaneously incubated with the enzyme labeled detection antibody (specific to 39-84 aa residue) and a biotin coupled capture antibody (specific to 1-6 aa residue) in a streptavidin-coated microplate well. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of intact PTH in the sample. Standards are used to generate a dose response curve of absorbance unit vs. concentration. Concentrations of intact PTH present in the controls and patient samples are determined directly from this curve.

STORAGE AND STABILITY 
1. Store the kit at 2-8 C 
2. Keep microwells sealed in a dry bag with desiccants. 
3. The reagents are stable until expiration of the kit. 
4. Do not expose reagents to heat, sun, or strong light.